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cleancap reagent ag  (New England Biolabs)


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    Structured Review

    New England Biolabs cleancap reagent ag
    Cleancap Reagent Ag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cleancap+reagent+ag/HiScribe+T7+mRNA+Kit+with+CleanCap+Reagent+AG/pm42034633-460-11-14
    Average 96 stars, based on 142 article reviews
    cleancap reagent ag - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    In Vitro:

    Article Title: Type I interferon restricts mRNA vaccine efficacy through suppression of antigen uptake in cDCs.
    Article Snippet: The insert was isolated via gel purification using Gel/PCR DNA fragments extraction kits (IBI scientific cat IB47020). .. These fragments were used for in vitro mRNA transcription via mRNA Kit with CleanCap Reagent AG (NEB Cat No. E2080S) utilizing CleanCap for the 5’ cap and pseudo-uridine in place of uridine as per manufacturers protocol, and left overnight at 37°C. ..

    Article Title: Non-destructive transcriptomics via vesicular export
    Article Snippet: .. A poly-adenylated transcript encoding mGL was in vitro transcribed using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, E2080S). ..

    Article Title: A CRISPR-Cas13d cancer therapeutic enables selective elimination of uveal melanoma
    Article Snippet: .. Cas13d mRNA was synthesized via in vitro transcription (IVT) using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, #E2080S). .. The IVT template was generated from plasmid RfxCas13d-P2A-mCherry (Addgene #155305, same as above) using PCR primers to incorporate T7 promoters.

    Article Title: Non-destructive transcriptomics via vesicular export.
    Article Snippet: .. A poly-adenylated transcript encodingmGLwas in vitro transcribed using theHiScribe T7mRNAKit with CleanCap Reagent AG (NEB, E2080S). ..

    Article Title: Type I interferon restricts mRNA vaccine efficacy through suppression of antigen uptake in cDCs
    Article Snippet: The insert was isolated via gel purification using Gel/PCR DNA fragments extraction kits (IBI Scientific cat IB47020). .. These fragments were used for in vitro mRNA transcription via mRNA Kit with CleanCap Reagent AG (NEB Cat No. E2080S) utilizing CleanCap for the 5’ cap and pseudo-uridine in place of uridine as per manufacturers protocol, and left overnight at 37 °C. ..

    Synthesized:

    Article Title: A CRISPR-Cas13d cancer therapeutic enables selective elimination of uveal melanoma
    Article Snippet: .. Cas13d mRNA was synthesized via in vitro transcription (IVT) using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, #E2080S). .. The IVT template was generated from plasmid RfxCas13d-P2A-mCherry (Addgene #155305, same as above) using PCR primers to incorporate T7 promoters.

    Purification:

    Article Title: ABCG transporter knockouts alter integument and eye pigmentation with gene-specific effects on viability in butterflies and moths
    Article Snippet: This modified plasmid was then linearized with NcoI-HF, and purified using the DNA Clean & Concentrator-25 kit (Zymo Research). .. Around 500 ng of linearized template was used for transcription, poly-adenylation and capping using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs, #E2080S), and mRNA was purified using the MEGAClear Transcription Clean-Up Kit (Thermo Fisher Scientific). ..



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